日本精品一区,欧美高清videos高潮hd,欲香欲色,亚洲国产成人久久一区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動(dòng)物巨噬細(xì)胞>CRL-28433D4/21 豬肺泡巨噬細(xì)胞

3D4/21 豬肺泡巨噬細(xì)胞

簡(jiǎn)要描述:CRL-2843 3D4/21 豬肺泡巨噬細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-2843
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:4207

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

ATCC 細(xì)胞|細(xì)胞株|細(xì)胞系|腫瘤細(xì)胞|巨噬細(xì)胞

3D4/21 (ATCC® CRL-2843™)

Organism Sus scrofa, pig

Tissue  lung

Cell Type  macrophage macrophage (alveolar); immortalized with SV40 large T antigen transformed with pSV3-neo

Product Format  frozen

Morphology  macrophage

Culture Properties  adherent

Biosafety Level  2  [Cells contain SV40 viral DNA sequences]

Age  27 days

Gender  unknown

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Strain  Landrace

Applications

These porcine myelomonocytic cell lines may have a wide variety of applications in porcine virology and immunology Ref.



Storage Conditions  liquid nitrogen vapor phase

Derivation  The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid.


Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).

Virus Susceptibility  Bovine adenovirus 3

Classical swine fever virus , Classical swine fever virus

Human parainfluenza virus 3

Swinepox virus

Vesicular stomatitis New Jersey virus

Porcine adenovirus

Herpes simplex virus 1

African swine fever virus

Pseudorabies virus

Vaccinia virus

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Swine vesicular disease virus

Comments

The plasmid carries the genes for neomycin resistance and SV40 large T antigen.


A subpopulation of each cell line (3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844)) was positive, to varying degrees depending on the media formulation, for nonspecific esterase activity and phagocytosis.


Clone 3D4/21 can produce Bovine adenovirus type 3 (BAV-3) to markedly higher titers than clones 3D4/2 and 3D4/31.


Addition of DMSO improved the capability of clone 3D4/21 to replicate the field isolate of African swine fever virus (ASFV/Lillie) compared to the other clones.

Complete Growth Medium  RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%

Subculturing

Volumes used in this protocol are for 75cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 x 103 to 7 x 103 viable cells/cm2 is recommended.

Incubate cultures at 37°C. Subculture when cell concentration reaches between 3 x 105 and 4 x 105 cells/cm2.

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended

Medium Renewal: Two to three times weekly

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.


CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Cryopreservation

Complete growth medium supplemented with 5% (v

) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: 5% CO2 in air recommended























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
懂色aⅴ精品一区二区三区蜜月| 日本免费一区二区三区| 男女27报xxoo做爰高潮| 日韩av片| 色老头oldmanⅴideos| 久久精品无码一区二区三区免费 | 又硬又粗又长又爽免费看| 黄色片久久| 亚洲国产欧美日韩另类| 久久精品国产亚洲AV天海翼| 亚洲色欲综合一区二区三区| 精品国产福利久久久| 国产A∨天天免费观看美女| av无码精品一区二区三区三级| 激情内射亚州一区二区三区爱妻| 临夏县| 色呦呦国产| 高清国产MV视频在线观看| 九九久久99综合一区二区| 久久精品九九亚洲精品| 波多野结衣绝顶大高潮| 99久久婷婷国产一区二区| 国精品99久9在线 | 免费| 五月天天爽天天狠久久久综合| 亚洲AV无码专区日韩乱码不卡| 精品国产91乱码一区二区三区| 你懂的在线播放| 另类av| 国产欧美一区二区三区沐欲| 福建论坛| 日本在线天堂| 人妻99| 久久丝袜| 南宫市| 99精产国品一二三产区区别电影| 久久福利中文字幕一区二区的| 少妇极品熟妇人妻无码| 国产精品成人免费视频一区| 上栗县| 羞羞影院午夜男女爽爽免费| 漾濞|