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MDA-kb2細胞， 人乳腺細胞

簡要描述：MDA-kb2細胞， 人乳腺細胞
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  • 產品型號：CRL-2713
  • 廠商性質：生產廠家
  • 更新時間：2024-11-15
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MDA-kb2細胞， 人乳腺細胞

ATCC® Number:CRL-2713™ Price:
Designations:MDA-kb2

Depositors:VS Wilson

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: mammary gland; breast
Cell Type: epithelial

Cellular Products:luciferase ( [57526] The cell line expresses firefly luciferase under control of the MMTV promoter that contains response elements for both glucocorticoid receptors (GR) and androgen receptors (AR).)

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
MDA-kb2細胞， 人乳腺細胞

Isolation:Isolation date: November 25, 1998

Applications:assay of androgen receptor ( [57526] MDA-kb2 may be used in an in vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.)

Receptors:androgen receptor [57526]

glucocorticoid receptor, positive [57526]



DNA Profile (STR):Amelogenin: X

CSF1PO: 10,12

D13S317: 12

D16S539: 9

D5S818: 11

D7S820: 10

THO1: 6

TPOX: 10

vWA: 17,18



Age:48 years adult

Gender:female

Ethnicity:Caucasian

Comments:Since both glucocorticoid receptor and androgen receptor are present in the MDA-MB-453 cells, and both receptors can act through the MMTV promoter, compounds that act through either androgen receptor or glucocorticoid receptor activate the MMTV luciferase reporter. Androgen receptor agonists such as dihydrotestosterone, and glucocorticoid receptor agonists such as dexamethasone, corticosterone, and aldosterone induce luciferase expression. Responsiveness of the cell line was monitored over time and was stable for more than 80 passages. The cell line is an in vitro tool for research in endocrine activity of the human androgen receptor and glucocorticoid receptor. It can be used to screen chemicals for both androgen receptor and glucocorticoid receptor mediated activities.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%


parental cell line:ATCC HTB-131



References:57526: Wilson VS, et al. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66: 69-81, 2002. PubMed: 11861974




















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