日本精品一区,欧美高清videos高潮hd,欲香欲色,亚洲国产成人久久一区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

簡要描述:CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-03-27
  • 訪  問  量:2102

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞 的詳細(xì)介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

ATCC® Number:CRL-1715™  Price:$329.00
Designations:TM4

Depositors:JP Mather

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:epithelial


Source:Organ: testis
Disease: normal
Cell Type: Sertoli cell;


Cellular Products:retinol binding protein

tissue plasminogen activator

transferrin



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Receptors:follicle stimulating hormone (FSH), expressed

androgen receptor, expressed

progesterone receptor, expressed



Tumorigenic:No

Antigen Expression:H-Y antigen; Mus musculus, expressed

Age:11 to 13 days

Gender:male

Comments:The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone (LH). The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid.

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: A 1:1 mixture of Ham'S F12 medium and Dulbecco's modified Eagle's medium with 1.2 g/L sodium bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell supension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:50 is recommended
Medium Renewal: Every 3 to 4 days


Preservation:Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040



References:1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781

1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561

1184: Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells. J. Biol. Chem. 259: 3117-3123, 1984. PubMed: 6538197

26150: Mather JP, Phillips DM. Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture. J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产成人精品福利网站| 免费特级黄毛片| 日韩成人极品在线内射3p蜜臀| 亚洲综合色丁香婷婷六月图片| china人妻呻吟ⅴideos| 国产精品一区二区av| 精品久久久久久久久久岛国| 日本高清视频在线WWW色| 成人无码一区二区三区网站| 中文一国产一无码一日韩| 亚洲欧洲日本精品专线| 宝山区| 人无娇妻中日久久持久久麻豆| chinesemature老熟女| 国产精品国三级国产av| 亚洲乱码中文字幕小综合| 波多野结衣AV高清中文字幕 | 日韩在线不卡| 成人在线免费播放| 国产馆av| 综合图区+亚洲+偷自拍| 韩国av| 久久性| 陆河县| 91精品国产91久久综合桃花| 999国际综州合另类| a级大黄片| 国产综合| 91麻豆精品秘密入口| 久久tv| 国产精品性| 男人的j插入女人的p| 四虎黄色影院| 国产av天堂| 97小视频| 久青草视频在线观看| 盗摄| 久久精品国产精品| 亚洲精品中文字幕乱码三区91| 日本av网站| www99热|