日本精品一区,欧美高清videos高潮hd,欲香欲色,亚洲国产成人久久一区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細胞庫>昆蟲卵巢細胞>CRL-1711 SF9 昆蟲卵巢細胞

CRL-1711 SF9 昆蟲卵巢細胞

簡要描述:CRL-1711 SF9 昆蟲卵巢細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2667

產(chǎn)品分類

Product Category

詳細介紹

CRL-1711 SF9 昆蟲卵巢細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件


CRL-1711 SF9 昆蟲卵巢細胞 的詳細介紹


ATCC® Number: CRL-1711™    Price: $275.00
Designations: Sf9

Depositors: G Smith, C Cherry, MD Summers

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:mixed: adherent/suspension

Organism:Spodoptera frugiperda (fall armyworm)

Morphology:epithelial
CRL-1711 SF9 昆蟲卵巢細胞


Source:Organ: ovary

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation: Isolation date: 1983

Applications:transfection host (Roche FuGENE® Transfection Reagents

technology from amaxa)



Virus Susceptibility:Nuclear polyhedrosis virus, Autographa californica

St. Louis encephalitis virus



Age: pupa

Gender: female

Comments:This line can be used to replicate baculovirus expression vectors.

It is important to use the medium described below.



Propagation: ATCC complete growth medium: Grace's Insect Medium with L-glutamine and supplemented with:

500 mg/L calcium chloride

2800 mg/L potassium chloride

3330 mg/L lactalbumin hydrolysate

3330 mg/L yeastolate

10% Heat-inactivated fetal bovine serum (previously tested for insect cell culture)


Atmosphere: air, 100%
Temperature: 28.0°C
Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.


Subculturing: Protocol: Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand (the latter is only preferable when working with larger flasks).
Subc*tion Ratio: A subc*tion ratio of 1:5 or greater is recommended
Medium Renewal: Every 2 to 3 days


Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


References:22650: Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13: 213-217, 1977. PubMed: 68913

23136: Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453

23294: Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519

32359: Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separay cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050

32361: Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063

32693: Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863

32860: Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109

32882: Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508

33023: Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802

33034: Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793

33143: Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda (Sf9) cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736

33160: Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久人人爽天天玩人人妻精品| 草莓视频香蕉视频| 国产日韩AV在线播放| 国产精品无码不卡一区二区三区 | 国产在线无码精品麻豆| 苍梧县| 国产国拍亚洲精品av在线 | 天天日天天爱| 国产精品亚洲一区二区三区在线观看| 精品国产高清Av自产拍在线| 亚洲无线一二三四区手机| 午夜天堂精品久久久久| 99久久99久久精品国产片| 被闺蜜的男人CAO翻了求饶| 精品亚洲成AV人在线观看| 夫妇交换聚会群4P大战图片| 精品日韩一区二区三区| 久久99亚洲精品久久| 欧美久| 欧美大黄片| 婷婷中文字幕| 在线av| 久久福利网| www.日| 九色国产精品入口| av最新地址| 久久一区二区三区精华液介绍| 免费无码黄网站在线看| 国产精品成人观看视频| 黄网站色成年片大免费高清| 亚洲中文字幕无码爆乳app| 自拍偷自拍亚洲精品10p| 亚洲AV成人片无码| 国产成人综合亚洲亚洲国产第一页| 国产又大又硬又粗| 国产精品宾馆在线精品酒店| 色综合久久婷婷88| 精品无码人妻一区二区三区| 色偷偷88888欧美精品久久久| 色婷婷亚洲一区二区三区| 天堂av亚洲av一二三区|